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RSS (file format), Really Simple Syndication, Rich Site Summary or RDF Site Summary, several similar forms of web syndication used by news websites and weblogs

Radio Service Software, a proprietary suite of programs sold by Motorola

Relay Spam Stopper, a Spam Blacklist run by Mail-abuse.org*, operated by MAPS (owned by Trend Micro)

Resident Set Size or Resident Segment Size, a measurement of RAM usage; represents actual physical RAM being used

Random Shutdown Syndrome, a phenomenon affecting MacBook notebooks which overheat and shut down without warning

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Reduced Space Symbology, a type of barcode developed by the GS1 US

Rotating Service Structure, used in the Space Shuttle program launches

Range Safety System, used in the Space Shuttle Solid Rocket Booster and other systems

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Recombination signal sequences, in immunological genetics, direct somatic recombination of gene segments

Residual sum of squares, in regression models

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Repetitive strain injury, also Repetitive stress syndrome, an overuse syndrome

Russell-Silver syndrome

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Rashtriya Swayamsevak Sangh, a right wing Hindu nationalist organization in India.

Recruiting Sub-Station, a satellite office of a main military recruiting station.

Regional Spatial Strategy provides a regional level planning framework for the regions of England

Regional Security System, an agreement between several countries situated in the Eastern Caribbean region. The RSS acts as a unit made up of a series of defence and disaster management forces.

Religious and Social Studies, a subject offered in many secondary schools in the United Kingdom

Royal Statistical Society, the main professional body for statisticians in the UK

Rural Sociological Society

Rochester Subway, which had the AAR reporting mark, RSS

Rockdale, Sandow and Southern Railroad, which currently has the AAR reporting mark RSS

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Rastriya Samachar Samiti, the national news agency of Nepal.

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Red Swastika School, Singapore