A "mirror Enterprise" appears in The Original Series episode "Mirror, Mirror". The ship in almost every way appears as an exact duplicate of the Federation starship USS ''Enterprise''. The clearest difference, the nearly omnipresent logo of the Terran Empire – was a knife stabbing through a stylized Earth – seen on doors and bulkheads throughout the ship. Some interior locations are darker and filled with devices of punishment and torture, such as the agony booth and the mirror Captain Kirk's deadly Tantalus device.

The Star Trek: Enterprise episode "In a Mirror, Darkly" features a Mirror Universe ship that in almost every way appears as an exact duplicate of the Enterprise. Differences include the use of a cloaking device captured from the Suliban, escape pods, a tractor beam as opposed to a grappler, a torture booth created by the mirror Doctor Phlox and Malcolm Reed, and different exterior markings. It is commanded by Captain Maximilian Forrest, although for a brief time his first officer, Commander Jonathan Archer, takes command following a mutiny. This Enterprise is destroyed by the Tholians.